Study Set-up : Better Data Driving Better Decisions

Study Set-up

Once the animal is recovered, there are still some tips that will help you have a successful study. Learn more in this section.

Can I infuse substances or molecules through a cOFM probe into the brain in vivo? What is the infusion factor?

Yes, cOFM probes can also be used as a tool for infusion of substances. For simple infusion (push only), the infusion factor is 100%. If the substance or mol...

Fri, 3 Aug, 2018 at 11:58 AM

Do I need to include BSA (Bovine Serum Albumin) in the perfusate to minimize non-specific binding? What percentage do you recommend?

The perfusate composition is crucial for successful cOFM sampling. We suggest performing an in vitro adsorption test prior to the in vivo sampling to assess ...

Fri, 3 Aug, 2018 at 12:28 PM

We are interested in sampling in awake animals at different time points over several days (maybe two samples per day), but we do not need to use the perfusate for the entire period. Is it necessary for the pump to be continuously operated b

The cOFM sampling insert can be replaced with the healing dummy after sampling if you are not continuously perfusing the cOFM system. Leaving a sampling in...

Fri, 3 Aug, 2018 at 12:37 PM

I understand that the pump can be used for up to one month of continuous sampling, but how long can I continuously perfuse in vivo? Is there any evidence of tissue reactivity caused by the pump liquid flow into the cOFM probe/brain?

Typical single continuous sampling sessions last from 24 hours to 5 days. There is no evidence of tissue reactivity. Please contact BASi, for more informati...

Fri, 3 Aug, 2018 at 12:39 PM

When I change the healing dummy against the sampling insert, is there a run-in period like with microdialysis where the membrane has to swell?

Unlike microdialysis, no membrane swelling is necessary. However, we suggest to perform a run-in phase at the sampling flow rate for at least one hour befor...

Fri, 3 Aug, 2018 at 2:29 PM

How is it possible to do perform these techniques in an awake animal with such small pieces?

For implantation animals have to be anesthetized with appropriate anesthetics (e.g. isoflurane or ketamine) for 30 to 45 min. Then the BBB must be allowed t...

Mon, 29 Oct, 2018 at 4:01 PM

I found air bubbles in my tubing set. What can I do against it?

Refer to the picture or attachment below. There are 3 possible causes that air bubbles can enter the tubing system: 1.) The Guide was not properly attached...

Mon, 29 Oct, 2018 at 4:09 PM