The cOFM sampling insert can be replaced with the healing dummy after sampling if you are not continuously perfusing the cOFM system.  Leaving a sampling insert in the guide without continuous pumping may lead to clogging. cOFM sampling can be restarted on the next sampling day by following the protocol in the user manual. This procedure can be repeated as long the cOFM probe and animal are in good condition. We recommend one day of rest for the experimental animal to recover. For every sampling session, use a new locking wedge to secure the sampling insert and use new tubing systems to guarantee best sampling quality.