Flush mode is generally used to flush the tubing set and get rid of air bubbles. Thus the pump is operated at a freely selectable flowrate of e.g. 10 µl/min. The flush mode allows you to select the amount of time and the flowrate at which the system will be flushed and what happens after the time has elapsed e.g. "Auto Pump", "Confirm Pump, "Auto Stop", "Confirm Stop". Please refer to the OFM pump user manual which is also available on the BASi webpage (Resources & Support). Chapter “Menu Structure, Settings and Navigation”.
The second flush is identical to first flush mode but allows you to set different parameters. This might be necessary for some experiments.
In general we recommend to flush the tubing set until it is air bubble free and afterwards to perfuse it at the desired flow rate of e.g. 0.3 to 1.0 µl/min.