How do you prevent liquid from coming out of the connectors on the perfusate bag when filling with a syringe? : Better Data Driving Better Decisions

How do you prevent liquid from coming out of the connectors on the perfusate bag when filling with a syringe? Print

Modified on: Mon, 29 Oct, 2018 at 3:36 PM

Please refer to the OFM pump user manual which is also available on the BASi webpage (Resources & Support). Chapter “Installing Pump Tubing and Perfusate Bag” shows step by step instructions on how to install and fill the perfusate bag without air bubble.

1. Unpack tubing set and perfusate bag.

2. Remove red protection cap of perfusate bag by turning it counter clock wise. DO NOT remove injection port.

3. Connect perfusate bag with the push tubing by turning the luer-connector clock wise.

4. Fill the perfusate bag with a (sterile) syringe via the injection port.

CAUTION:

DO NOT pierce perfusate bag with the needle. Risk of contamination.

DO NOT use aggressive fluids (alcohols, acids, bleaches, etc.) and hot fluids (>45°C) as perfusate (see instructions for use of perfusate bag).

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How do you prevent liquid from coming out of the connectors on the perfusate bag when filling with a syringe? Print

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