No, the tubing does not have to be switched every time. The tubing sets are gamma irradiated but biofouling can still occur after using the tubing set for a period of time, especially under unsterile conditions. Furthermore, tubing sets wear out over time, but can be typically used for 72 hours. If tubing sets are used longer, check the flow rate by measuring sampling time and weight and calculate the actual flow rate (flow (µl/min) = weight (µl) / time (min). If the tubing set is not used for a couple of days between two sampling sessions, open the pump head and remove it from the pump. If multiple sampling sessions in experimental animals are performed, we recommend one day of rest for the experimental animal to recover. For every sampling session, use a new locking wedge to secure the sampling insert and use new tubing systems to guarantee the best sampling quality.
Does the entire tubing apparatus need to be switched after every use? In the past, I have used the same tubing sets for 5 continuous days (usually 3 to 4 studies that are 24 hours long with overnight stabilization and a buffer flush for a f Print
Modified on: Fri, 3 Aug, 2018 at 12:45 PM
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